Desenvolvimento somÃtico e sensÃrio-motor e padrÃo do consumo alimentar, em ratos: efeitos do tratamento com inibidor de recaptaÃÃo da serotonina durante o perÃodo de crescimento rÃpido do encÃfalo

AUTOR(ES)
DATA DE PUBLICAÇÃO

1998

RESUMO

It was investigated in this work the influences of the treatment with selective serotonin reuptake inhibitor, over the reflex-ontogeny, somatic maturation and adult pattern of food intake. Male Wistar rats were divided in groups, receiving daily, from 1st to 21st day of life, the following treatments, s.c.: Ingenuous (Ing, n=26), any treatment; Saline (Sal, n=24), NaCl 0.9% (2 mg/kg); and Citalopram (Cit), in three doses: 5 mg/kg (Cit5, n=10); 10 mg/kg (Cit10, n=10); 20 mg/kg (Cit20, n=26). It was evaluated daily, from 1st to 21st day, and every 3 days, from 24th to 60th day P.N., the following: body weight (BW), tail extent (TE), lateral axis of the head (LAH), and fore back axis of the head (FBAH). Daily, it was recorded the day of appearance of the following physical features: opening of the eyes (OE), incisor eruption (IE), opening of auditory duct (OAD) and ear unfolding (EU). Daily, the following reflexes were evaluated: palmar grasp (PG), righting (R), cliff avoidance (CA), negative geotaxis (NG), auditory startle (AS) and free-fall righting (FFR). By 70-80 days of age, the animals were submitted to a food-intake study, during seven days, through the evaluation of: absolute corporal weight (ACW), absolute and relative weight change (AWC; RWC), absolute and relative food intake (AFI; RFI), absolute and relative water intake (AWI; RWI), absolute and relative fecal excretion (AFE; RFE), absolute and relative urinary excretion (AUE; RUE) and the ratio AWI/QUE. By 110-120 the rats were killed and some organs removed to record their weight: Brain â absolute and relative wet brain weight â AWBW; RWBW â and absolute and relative dry brain weight â ABBW, RDBW, Cerebellum (absolute and relative cerebellum wet weight) AWCW, RWCW and absolute and relative cerebellum dry weight (ADCW, RDCW), and Liver (absolute and relative wet weight â AWLW, RWLW, and absolute and relative dry weight â ADLW, RDLW). The statistic analysis was made with ANOVA followed by Tukeyâs test (parametric data) and Kruskal-Wallis followed by Mann-Whitney test (non-parametric data). There was no difference between Ing and Sal in all the parameters, so, Sal was chosen as control. Murinometric evaluation: During lactation, there was a reduction (p<0.01), after the following days of age (until 21st) on BW on Cit10=15th; Cit20=6th; TE: Cit20=9th; LAH: Cit10=15th, Cit20=9th; FBAH: Cit20=15th. During the 24th until 60th period, there was a reduction (p<0.01) of: BW: Cit10=48th until 60th day; Cit20=24th until 60th day; TE: Cit20=24th until 60th; LAH: Cit10=24th until 44th; Cit20=24th until 60th; FBAH: Cit20=34th until 60th. Therefore, Cit5 had higher BW than Sal from 45th until 60th day. Physical Features Maturation: There was a later maturation of: OAD in Cit5 and Cit20 (p<0.05) and III in Cit5 and Cit10 (p<0.05). Sensory motor development: The following reflexes were delayed (p<0.01): PG, R, VP and FFR in all citalopram groups: AS in Cit5, Cit10 and Cit20; CA in Cit20; NG in Cit5 and Cit20. Food intake: There was a reduction (p<0.01) on: ABW in Cit20; AFI on Cit5, Cit10 and Cit20; AFE on Cit10 and Cit20; QUE on Cit20. However, there was an increase (p<0.01) of RWI and AWI/QUE on Cit20. There was no difference about RWC, AWC, FRI and RFE between any treatment group, compared do SAL. Organ weight: There was a decrease (p<0.01) of: AUBW, ADBW and AWCW in Cit20. However, RDBW were higher in Cit20, compared to SAL. AWLW decreased in Cit10 and Cit20; ADLW decreased in Cit10 and Cit20. Chronic treatment with SSRI during lactation period delays ontogeny reflex and shifts the somatic (physical) maturation in rats. Is also was seen shifts on the weight gain until adulthood. At this time, sequels seen in the organs and body weight seem to be associated to serotonergic system manipulation. However, some unspecific effects of SSRI must not be rejected. In adulthood, some shifts were found in some parameters of food intake. In a general way, the shifts are related to the employed dose. These suggest a participation of different kinds of serotoninergic of receptors. Studies about pharmacologic manipulations with specific serotonergic agonists and antagonists are still necessary. Nevertheless, the results are likely to confirm that the serotoninergic system plays an important role on somatic and sensory motor development on adult pattern of food intake

ASSUNTO(S)

dieta equilibrada - ratos aleitamento materno - ratos - comportamento neonatal e adulto serotonina â sistema nervoso central (snc) â antidepressivos - ratos nutricao crescimento - reflexos e medidas murinomÃtricas

ACESSO AO ARTIGO

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