Desenvolvimento e validação de protocolos para a anotação automática de sequências ORESTES de Eimeria spp. de galinha doméstica. / Development and validation of protocols for automated annotation of ORESTES sequences of Eimeria spp. of domestic fowl.
AUTOR(ES)
Milene Ferro
DATA DE PUBLICAÇÃO
2008
RESUMO
Avian coccidiosis is an enteric disease caused by protozoan parasites of the genus Eimeria. Aiming at obtaining a better understanding of the molecular mechanisms that regulate the life cycle of the parasites, our group generated 15,000 expressed sequences (ORESTES) for each one of the three most important species: E. tenella, E. maxima and E. acervulina. In the present work, we report the development of a set of components for the automated sequence annotation through EGene, a platform for pipeline construction previously described by our group (Durham et al. Bioinformatics 21: 2812-2813, 2005). These components were used to construct pipelines for the automated annotation of assembled sequences of ORESTES of Eimeria spp. The annotation process consisted in the identification of genes and the corresponding protein function based on a set of evidences. The sequences were also mapped and quantified using a controlled vocabulary of gene ontology (GO) terms.
ASSUNTO(S)
bioinformática expressed sequences tags processo encadeado pipeline sequence annotation bioinformatics eimeria spp. seqüência de dna anotação de sequências biológicas etiquetas de seqüencias expressas eimeria spp. dna sequences
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