Descrição espermática do sêmen de rã-pimenta (Leptodactylus labyrinthicus) / Description of semen of sperm-pepper frog (Leptodactylus labyrinthicus)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

This study was developed at Ranário Experimental in Universidade Federal de Viçosa, Viçosa (MG) from April 6th, 2009 to June 7th, 2009, and aimed to assess the seminal characteristics and analyze the morphology of semen and sperm peppermint frog (Leptodactylus labyrinthicus). We collected eight male rã-pimenta, suitable for reproduction, in Viçosa, Cajuri, Timóteo and Governador Valadares. The assumed temperature was 25.0 1.5 C with two photoperiods: LD 12:12 (LD = Light Dark) and 14:10 LD. For induction of spermiogenesis, we used the hormone analogue buserelin acetate (GnRHa) at a dose of 0.4 mg (= 0.1 mL), charged in the coelomic cavity of the animal with the aid of a hypodermic needle 25, 0 x 0.7 mm. This procedure was performed for two consecutive days: five doses on the first day with an hour interval between doses, and three doses in the second, with the first dose at the same time the day before and 1 h interval between doses. On the first day were collected five of the semen specimens of frog pepper (one, two, three, four and five hours after the first hormone application). To verify the effect of treatment was used an optical microscope with bright field (400x increase), which examined slides with semen. It was considered positive the slides with the presence of spermatozoa. An aliquot of fresh semen from each animal, corresponding to a value of one drop (approximately 0.05 mL) was placed on a glass slide and the tax rate, a cover slip. Force, motility and sperm concentration were avaluated. The animals responded positively to the hormone only the second day (5 h of treatment the first day and after 24 h, two more hours of treatment), totaling 26 h to collect semen, photoperiod of 14: 10 LD. In Plant Anatomy Laboratory, Universidade Federal de Viçosa, we used the light microscope Olympus BX-40 and held image capture, which was used for the analysis of 28 sperm, with the help of the program analyzes the image IMAGE PRO PLUS, the head area and total length, head, tail and the membrane surrounding the head. The relevant time minimum-maximum (average) were: the head area: 15.57 to 50.15 (29.80) μm2, total length: 26.89 -76.06 (21.21) μm and the membrane surrounding the head.

ASSUNTO(S)

rã-pimenta morfologia sperm morphology semen pepper frog sêmen morfologia espermática

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