Derris (Lonchocarpus) urucu (Leguminosae) Extract Modifies the Peritrophic Matrix Structure of Aedes aegypti (Diptera:Culicidae)

AUTOR(ES)

Gusmão, Desiely Silva, Páscoa, Valéria, Mathias, Leda, Vieira, Ivo José Curcino, Braz-Filho, Raimundo, Lemos, Francisco José Alves

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2002-04

RESUMO

Aqueous suspension of ethanol extracts of Derris (Lonchocarpus) urucu (Leguminosae), collected in the state of Amazonas, Brazil, were tested for larvicidal activity against the mosquito Aedes aegypti (Diptera:Culicidae). The aim of this study was to observe the alterations of peritrophic matrix in Ae. aegypti larvae treated with an aqueous suspension of D. urucu extract. Different concentrations of D. urucu root extract were tested against fourth instar larvae. One hundred percent mortality was observed at 150 µg/ml (LC50 17.6 µg/ml) 24 h following treatment. In response to D. urucu feeding, larvae excreted a large amount of amorphous feces, while control larvae did not produce feces during the assay period. Ultrastructural studies showed that larvae fed with 150 µg/ml of D. urucu extract for 4 h have an imperfect peritrophic matrix and extensive damage of the midgut epithelium. Data indicate a protective role for the peritrophic matrix. The structural modification of the peritrophic matrix is intrinsically associated with larval mortality.

Documentos Relacionados

• Determinação da variabilidade genética de populações de Aedes aegypti (diptera:culicidae) utilizando marcadores de rapd.
• Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
• AvaliaÃÃo de um biolarvicida à base de Bacillus thuringiensis sorovar. israelensis, desenvolvido no Brasil, para o controle do Aedes aegypti (Diptera:Culicidae)
• Reproductive aspects of the mosquito Culex quinquefasciatus (Diptera:Culicidae) infected with Wuchereria bancrofti (Spirurida: Onchocercidae)
• Flavonoides de Lonchocarpus campestris (Leguminosae)