Derivatization of urinary benzolecgonine with diazomethane to verify cocaine exposure using chromatographic techniques / Derivação de benzoilecgonina urinária com diazometano para verificação da exposição à cocaína por técnicas cromatográficas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2000

RESUMO

Cocaine abuse is now representing one of the greatest world public health problem. Great social interests have been generated with the using of toxicological analyses with the aim of detecting cocaine exposure to adopt prevention and control measures. Benzoylecgonine, the main metabolite found in urine, is one of the biological markers of cocaine exposure. However, its physical-chemical properties make the chromatographic analyses of this substance a difficult task. Liquid-liquid extraction of this analyte from biological sample does not provide good recovery and the derivatization of benzoyleconine for further detection by gas chromatography is a need. In this study, the application of solid phase extraction followed by benzoylecgonine conversion into cocaine with diazomethane made the standardization of the method possible. The following techniques were used: gas chromatography/ mass spectrometry (GC/MS), gas chromatography/ nitrogen-phosphorous detection (GC/NPD) and high performance thin-Iayer chromatography (HPTLC). Samples collected from cocaine abusers were submitted to the standardized extraction and derivatization techniques. Cocaine was detected in ali samples when chromatography was applied.

ASSUNTO(S)

benzoylecgonine urina benzoilecgonina cocaine cromatography cocaína cromatografia urine

ACESSO AO ARTIGO

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