Derivative spectrophotometric method for determination of acyclovir in polymeric nanoparticles
AUTOR(ES)
Tavares, Guilherme Diniz, Ishikawa, Gisele Miki, Monteiro, Talita Ferreira, Zanolini, Carolina, Kedor-Hackmann, Érika Rosa Maria, Bou-Chacra, Nádia Araci, Consiglieri, Vladi Olga
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2012
RESUMO
A derivative spectrophotometric method was validated for quantification of acyclovir in poly (n-butylcyanoacrylate) (PBCA) nanoparticles. Specificity, linearity, precision, accuracy, recovery, detection (LOD) and quantification (LOQ) limits were established for method validation. First-derivative at 295.2 nm eliminated interferences from nanoparticle ingredients and presented linearity for acyclovir concentrations ranging from 1.25 to 40.0 µg/mL (r = 0.9999). Precision and accuracy data demonstrated good reproducibility. Recovery ranged from 99.3 to 101.2. LOD was 0.08 µg/mL and LOQ, 0.25 µg/mL. Thus, the proposed method proved to be easy, low cost, and accurate, and therefore, an useful alternative to quantify acyclovir in nanoparticles.
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