Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli.
AUTOR(ES)
Ohnishi, T
RESUMO
Freeze-drying of Escherichia coli cells caused strand breaks of deoxyribonucleic acid (DNA) in both radiation-sensitive and -resistant strains. However, in the radiation-resistant strain E. coli B/r the damaged DNA was repaired after rehydration, whereas in the radiation-sensitive strain E. coli Bs-1 the damaged DNA was not repaired and the DNA was degraded. Repeated freeze-drying did not break the damaged DNA into smaller pieces.
