Dehydration-Stress-Regulated Transgene Expression in Stably Transformed Rice Plants1

AUTOR(ES)

Su, Jin

FONTE

American Society of Plant Physiologists

RESUMO

To confer abscisic acid (ABA) and/or stress-inducible gene expression, an ABA-response complex (ABRC1) from the barley (Hordeum vulgare L.) HVA22 gene was fused to four different lengths of the 5′ region from the rice (Oryza sativa L.) Act1 gene. Transient assay of β-glucuronidase (GUS) activity in barley aleurone cells shows that, coupled with ABRC1, the shortest minimal promoter (Act1–100P) gives both the greatest induction and the highest level of absolute activity following ABA treatment. Two plasmids with one or four copies of ABRC1 combined with the same Act1–100P and HVA22(I) of barley HVA22 were constructed and used for stable expression of uidA in transgenic rice plants. Three Southern blot-positive lines with the correct hybridization pattern for each construct were obtained. Northern analysis indicated that uidA expression is induced by ABA, water-deficit, and NaCl treatments. GUS activity assays in the transgenic plants confirmed that the induction of GUS activity varies from 3- to 8-fold with different treatments or in different rice tissues, and that transgenic rice plants harboring four copies of ABRC1 show 50% to 200% higher absolute GUS activity both before and after treatments than those with one copy of ABRC1.

Documentos Relacionados

• Differential Expression of Genes for Cyclin-Dependent Protein Kinases in Rice Plants1
• Salt Stress Activation of Wound-Related Genes in Tomato Plants1
• BRIEF PAPERS: EFFECT OF VITAMIN B1 ON THE GROWTH OF RICE PLANTS1
• Induction of Lipid Metabolic Enzymes during the Endoplasmic Reticulum Stress Response in Plants1
• The Role of Iron-Deficiency Stress Responses in Stimulating Heavy-Metal Transport in Plants1