Defective transcription factor activation for proinflammatory gene expression in poly(ADP-ribose) polymerase 1-deficient glia
AUTOR(ES)
Ha, Hyo Chol
FONTE
National Academy of Sciences
RESUMO
Poly(ADP-ribose) polymerase 1 (PARP-1) activity is detected in both neuronal and nonneuronal cells in the CNS, and excessive PARP-1 activity is known to be detrimental to tissue because of the cellular energy loss. Accordingly, PARP-1-deficient (PARP-1-/-) mice have been shown to be resistant to cerebral ischemia and several forms of inflammation. Recently, PARP-1 in glial cells has been shown to mediate the expression of proinflammatory genes in response to inflammatory stimuli by, in part, enhancing cognate DNA-binding capacities of transcription factors such as NF-κB and activator protein 1. Here, we demonstrate an additional mechanism whereby a significant reduction of proinflammatory gene expression such as IL-1β, tumor necrosis factor α, and inducible nitricoxide synthase in PARP-1-/- glial cells is linked to defective inflammatory stimuli-induced p38MAPK-mediated phosphorylation of ATF-2 and cAMP-response element-binding protein and phosphorylation of NF-κB p65. Importantly, an inflammatory stimuli-induced p38MAPK activation is impaired in PARP-1-/- glial cells in a signaling pathway- and cell/tissue type-specific manner. These findings indicate that PARP-1 is an essential host factor among factors that actively mediate excessive production of proinflammatory molecules in glial cells, which may in turn contribute to the initiation of neuronal injuries.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=387378Documentos Relacionados
- Poly(ADP-ribose) polymerase-1 dependence of stress-induced transcription factors and associated gene expression in glia
- Decreased expression of topoisomerase IIbeta in poly(ADP-ribose) polymerase-deficient cells.
- Poly(ADP-ribose) polymerase enhances activator-dependent transcription in vitro
- Activation of the abundant nuclear factor poly(ADP-ribose) polymerase-1 by Helicobacter pylori
- DNA repair defect in poly(ADP-ribose) polymerase-deficient cell lines.