Decreased expression of heat shock protein 70 mRNA and protein after heat treatment in cells of aged rats.
AUTOR(ES)
Fargnoli, J
RESUMO
The effect of aging on the induction of heat shock protein 70 (HSP70)-encoding gene expression by elevated temperatures was studied in cultures of lung- or skin-derived fibroblasts from young (5 mo) and old (24 mo) male Wistar rats. Although the kinetics of the heat shock response were found to be similar in the two age groups, we observed lower levels of induction of HSP70 mRNA and HSP70 protein in confluent primary lung and skin fibroblast cultures derived from aged animals. Additional experiments with freshly excised lung tissue showed a similar age-related decline in the heat-induced expression of HSP70.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=53363Documentos Relacionados
- Cloning of the Heat Shock Protein 70 (HSP70) Gene of Ehrlichia sennetsu and Differential Expression of HSP70 and HSP60 mRNA after Temperature Upshift
- Recovery of protein synthesis after heat shock: prior heat treatment affects the ability of cells to translate mRNA.
- Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation in rat fibroblasts.
- Constitutively expressed rat mRNA encoding a 70-kilodalton heat-shock-like protein.
- Posttranscriptional regulation of hsp70 expression in human cells: effects of heat shock, inhibition of protein synthesis, and adenovirus infection on translation and mRNA stability.