"DAKLI": a multipurpose ligand with high affinity and selectivity for dynorphin (kappa opioid) binding sites.
AUTOR(ES)
Goldstein, A
RESUMO
We describe a synthetic ligand, "DAKLI" (Dynorphin A-analogue Kappa LIgand), related to the opioid peptide dynorphin A. A single reactive amino group at the extended carboxyl terminus permits various reporter groups to be attached, such as 125I-labeled Bolton-Hunter reagent, fluorescein isothiocyanate, or biotin. These derivatives have high affinity and selectivity for the dynorphin (kappa opioid) receptor. An incidental finding is that untreated guinea pig brain membranes have saturable avidin binding sites.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=282189Documentos Relacionados
- Deltorphins: a family of naturally occurring peptides with high affinity and selectivity for delta opioid binding sites.
- Interleukin 3 activates human blood basophils via high-affinity binding sites.
- Primary structure and functional expression of a guinea pig kappa opioid (dynorphin) receptor.
- Binding of actinomycin D to DNA: evidence for a nonclassical high-affinity binding mode that does not require GpC sites.
- Separation of two thyrotropin binding components from porcine thyroid tissue by affinity chromatography: characterization of high and low affinity sites.
