Cytotoxic activity of the chloroform extract and four diterpenes isolated from Salvia ballotiflora

AUTOR(ES)

Campos-Xolalpa, Nimsi, Alonso-Castro, Ángel Josabad, Sánchez-Mendoza, Ernesto, Zavala-Sánchez, Miguel Ángel, Pérez-Gutiérrez, Salud

FONTE

Rev. bras. farmacogn.

DATA DE PUBLICAÇÃO

2017-06

RESUMO

Abstract New compounds with chemotherapeutic activity are sought after, and plants are an important source of these compounds. Four diterpenes, 19-deoxyicetexone, 7,20-dihydroanastomosine, icetexone and 19-deoxyisoicetexone, were isolated from the hexane-washed chloroform extract of Salvia ballotiflora. The cytotoxic activity of the hexane-washed chloroform extract and its four diterpenes were tested using the MTT assay against three tumor cell lines: HeLa (cervical cancer), A549 (lung cancer) and MCF7 (breast cancer), and two murine cell line: J774A.1 (epithelial cancer) and CT26 (colon cancer), and their IC50 values were determined. 19-Deoxyisoicetexone had the greatest effect on HeLa cells with IC50 of 3.2 µg/ml (9.36 µM), whereas hexane-washed chloroform extract had the best cytotoxic effect on A549 cells with an IC50 of 2.29 µg/ml. These effects of 19-deoxyisoicetexone and hexane-washed chloroform extract were with similar activity compared to cisplatin (IC50 = 1.06 µg/ml in HeLa cells, and 4.6 µg/ml (15.21 µM) in A549 cells).

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