Cytoplasmic RNA modulators of an inside-out signal-transduction cascade

AUTOR(ES)

Blind, Michael

FONTE

The National Academy of Sciences

RESUMO

A vaccinia virus-based RNA expression system enabled high-level cytoplasmic expression of RNA aptamers directed against the intracellular domain of the β2 integrin LFA-1, a transmembrane protein that mediates cell adhesion to intercellular adhesion molecule-1 (ICAM-1). In two different cell types, cytoplasmic expression of integrin-binding aptamers reduced inducible cell adhesion to ICAM-1. The aptamers specifically target, and thereby define, a functional cytoplasmic subdomain important for the regulation of cell adhesion in leukocytes. Our approach of aptamer-controlled blocking of signaling pathways in vivo could potentially be applied wherever targeted modulation of a signal-transduction cascade is desired.

Documentos Relacionados

• The structure of an integrin/talin complex reveals the basis of inside-out signal transduction
• Bacteriorhodopsin is an inside-out protein.
• Glutathione transport by inside-out vesicles from human erythrocytes.
• Inside-out model for self-assembly of tobacco mosaic virus.
• Activation of a paramyxovirus fusion protein is modulated by inside-out signaling from the cytoplasmic tail