Cytochrome c. Observation of Numerous Single-Carbon Sites of the Reduced and Oxidized Species by Means of Natural-Abundance 13C Nuclear Magnetic Resonance Spectroscopy

AUTOR(ES)

Oldfield, Eric

RESUMO

Proton-decoupled, natural-abundance 13C nuclear magnetic resonance spectra (obtained at 15.18 MHz by the Fourier transform method) of aqueous ferrocytochrome c, ferricytochrome c, and mixtures of both species were recorded. The 18 nonprotonated aromatic carbons of amino-acid residues and the 16 nonprotonated aromatic carbons of the heme yielded 22 narrow single-carbon resonances and 6 narrow two-carbon resonances in the spectrum of ferrocytochrome c. Only some of these resonances were detected in the spectrum of ferricytochrome c.

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