Cultivo in vitro, crescimento e floração in vivo de especies de Clusia L. (Guttiferae)

AUTOR(ES)
DATA DE PUBLICAÇÃO

1999

RESUMO

The genus Clusia L. (Guttiferae) is characterized by species that produce secondary metabolic compounds of recognized biological activity. An attempt was made to identify patterns of physiological response related to the compound production in order to facilitate their study and haracterization. This study investigated two physiological aspects of Clusia species: 1) the establishment of a standardized protocol for in vitro culture of native speciesi 2) the influence of photoperiod on flowering in C. nemorosa and C. fluminensis. C lusia nemorosa was choosen for n vi tro culture experiments. Germination studies under controlled conditions made it possible to obtain young plant material with easy asepsis. The young leaves of plants germinated in the laboratory and rown in a greenhouse were the best explant source for in vitro culture. Combinations of plant growth regulators intended to induce callus formation in three species were tested, allowing the selection of combinations wi th two distinct purposes: NAA x BA, capable of. producing callus containing exudate nd 2,4-D x BA, to produce friable callus. Root culture in liquid medium was favoured in B5 medium with 2.0mg/L of NAA. Photoperiod studies showed C. nemorosa to be along-day plant with acritical daylength of about lOh. Although C. fluminensis plants did not flower in response to photoperiod treatments (day-neutral plant) , pattern of vegetative growth. They showed changes in the pattern of vegetative growth

ASSUNTO(S)

fotoperiodismo plantas medicinais

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