Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
AUTOR(ES)
Campos, Jacira Ribeiro, Rosa-e-Silva, Julio Cesar, Carvalho, Bruno Ramalho, Vireque, Alessandra Aparecida, Silva-de-Sá, Marcos Felipe, Rosa-e-Silva, Ana Carolina Japur de Sá
FONTE
Clinics
DATA DE PUBLICAÇÃO
2011
RESUMO
OBJECTIVE: To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozenthawed samples. METHODS: Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thawed and analyzed. Follicular density, follicular viability, and steroidogenic capacity were assessed. RESULTS: We observed no differences between the groups in follicular density, which was assessed in hematoxylin and eosin-stained tissue sections. A heterogeneous follicular distribution was observed in the parenchyma, with a mean density of 361.3±255.4, 454.9±676.3, and 296.8±269.0 follicles/mm3 for FG, G30 and G180, respectively (p = 0.46). Follicular viability was greater in FG (93.4%) when compared with the cryopreserved tissues (70.8% for G30 (p<0.001) and 78.4% for G180 (p<0.001)), with no difference in viability between the frozen samples (p>0.05). The steroidogenic capacity of the tissue was not significantly reduced following cryopreservation. CONCLUSION: The slow freezing procedures used for ovarian cryopreservation are capable of preserving follicular viability and maintaining the steroidogenic capacity of the tissue despite a roughly 30% decrease in follicular viability. Furthermore, short-term storage of ovarian tissue does not appear to compromise follicle integrity.
Documentos Relacionados
- Is caspase inhibition a valid therapeutic strategy in cryopreservation of ovarian tissue?
- Random-start controlled ovarian stimulation for emergency fertility preservation in a patient with myelodysplastic syndrome: a case report
- Follicular lesions of the thyroid. Does frozen section evaluation alter operative management?
- Ovarian Tissue Culture to Preserve Fertility in Transgender Male Patients after Hormonal Treatment
- Viability and Infectivity of Measles Virus-Infected Nervous Tissue Frozen in Cryoprotective Medium