Cryo-electron microscopy of vitreous sections
AUTOR(ES)
Al-Amoudi, Ashraf
FONTE
Nature Publishing Group
RESUMO
Since the beginning of the 1980s, cryo-electron microscopy of a thin film of vitrified aqueous suspension has made it possible to observe biological particles in their native state, in the absence of the usual artefacts of dehydration and staining. Combined with 3-d reconstruction, it has become an important tool for structural molecular biology. Larger objects such as cells and tissues cannot generally be squeezed in a thin enough film. Cryo-electron microscopy of vitreous sections (CEMOVIS) provides then a solution. It requires vitrification of a sizable piece of biological material and cutting it into ultrathin sections, which are observed in the vitrified state. Each of these operations raises serious difficulties that have now been overcome. In general, the native state seen with CEMOVIS is very different from what has been seen before and it is seen in more detail. CEMOVIS will give its full potential when combined with computerized electron tomography for 3-d reconstruction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=517607Documentos Relacionados
- Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles.
- The three-dimensional structure of reovirus obtained by cryo-electron microscopy.
- Cryo-electron tomography of vaccinia virus
- Subdomain organization of the Acanthamoeba myosin IC tail from cryo-electron microscopy
- Structure of a viral DNA gatekeeper at 10 Å resolution by cryo-electron microscopy