Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum

AUTOR(ES)

Pinheiro, Fábio, Palma-Silva, Clarisse, Barros, Fábio de, Cozzolino, Salvatore

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

27/03/2009

RESUMO

In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27 to 0.85, respectively. In addition we tested all loci in 35 species representative of the genus Epidendrum. Of these, 26 loci showed successful amplification. Cross-application of these loci represent a potential source of co-dominant markers for evolutionary, ecological and conservation studies in this important orchid genus.

Documentos Relacionados

• Description of novel microsatellite loci in the Neotropical fish Prochilodus argenteus and cross-amplification in P. costatus and P. lineatus
• Development and characterization of fifteen polymorphic microsatellite loci in Bryconamericus aff. iheringii (Teleostei: Characidae) and cross-amplification in related Characidae species
• CROSS-AMPLIFICATION OF MICROSATELLITE LOCI IN CRUSTACEA DECAPODA
• Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
• Novel Microsatellite Markers for Brazilian Mangrove Oysters ( Crassostrea gasar ) and their Cross-Amplification in Crassostrea rhizophorae