Crescimento e atividade de enzimas do metabolismo de nitrogenio em catiledones de Crotalaria juncea cultivados in vitro

AUTOR(ES)
DATA DE PUBLICAÇÃO

1997

RESUMO

An in vitro system for the growth of Crotalaria juncea L. was implemented. In order to study the metabolism of nitrogen, cotyledons collected 39 days after anthesis were grown for 8 days in a liquid culture medium containing different nitrogen sources. It was observed that the activities of the enzimes asparaginase, glutamine synthetase, glutamate syntase and glutamate dehydrogenase were greater when asparagine was used as a nitrogen source. Among the tested sources, glutamine provided the greatest growth rates and deposition of storage proteins, followed by asparagine. It was concluded that the lower efficiency obtained with asparagines can not be explained by a deficiency of the glutamine sinthetase activity, asparaginase limitation or glutamate insufficiency in the glutamine sinthetase reaction

ASSUNTO(S)

asparaginase nitrogenio - metabolismo crotalaria

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