Covalent attachment of synthetic DNA to self-assembled monolayer films.

AUTOR(ES)

Chrisey, L A

RESUMO

The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film formed on silica surfaces. A variety of aminosilanes, crosslinkers and treatment conditions have been tested to identify optimal conditions for DNA immobilization using this approach. The DNA films which result have been characterized using UV spectroscopy, water contact angle measurement, radiolabeling and hybridization methods.

Documentos Relacionados

• Zirconium phosphonate/1,4,5,8-naphthalenediimides self-assembled films
• Supramolecular self-assembled fullerene nanostructures
• Electrochemical modeling of electron and proton transfer to ubiquinone-10 in a self-assembled phospholipid monolayer.
• Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold.
• Emergent mechanical properties of self-assembled polymeric capsules