Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.

AUTOR(ES)

Wolfe, J

RESUMO

Clones containing sequences derived from the human Y chromosome have been isolated from cosmid libraries of a human-mouse hybrid cell line. These libraries were constructed in the new expression vectors Homer V and Homer VI. The collection of cosmids isolated is enriched for unique sequence DNA and only a few of the cosmids contain the tandemly repeated sequences which constitute a major portion of the Y chromosome. Three cosmids have been studied in detail. One cosmid shows extensive homology over at least 20 kb with the long arm of the X chromosome; this homology is outside the predicted homology region required for sex chromosome pairing. The other two clones contain unique sequences specific to the Y chromosome and both map to the heterochromatic region of the Y chromosome long arm.

Documentos Relacionados

• Distinctive patterns of histone H4 acetylation are associated with defined sequence elements within both heterochromatic and euchromatic regions of the human genome.
• The fourth chromosome of Drosophila melanogaster: Interspersed euchromatic and heterochromatic domains
• The human Y chromosome.
• Chromosome fragments with alphoid sequences derived from a pseudoisodicentric Y chromosome.
• Characterization and evolution of a single-copy sequence from the human Y chromosome.