Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay

AUTOR(ES)

Souza, Luiz Waldemar de Oliveira, Andrade, André Furugen Cesar, Celeghini, Eneiva Carla Carvalho, Negrão, João Alberto, Arruda, Rubens Paes de

FONTE

Revista Brasileira de Zootecnia

DATA DE PUBLICAÇÃO

2011-12

RESUMO

The objectives of this study were to validate a non-extractive RIA for seminal testosterone and quantify the hormone using a solid-phase commercial kit, and study the correlation between testosterone in seminal plasma and sperm characteristics. Parallelism showed a correlation index r = 0.992 (Y = -5.47 + 1.073X; R² = 0.985), indicating that the non-extractive method presented is indicated particularly for assessment of testosterone when establishing comparisons between samples. Overall mean (±SD) of testosterone level was 0.60±0.65 ng/mL. Correlation was only found between the seminal concentrations of testosterone and pH of the semen.

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