Contribuição ao estudo do acido folico em alimentos enriquecidos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2001

RESUMO

Despite the great impact of the results of new research in to possible beneficial effects of folic acid on the health, there are still not enough data about the content of folic acid in enriched food and no studies on its stability, chiefly in Brazilian foods. Therefore people are exposed to the consumption of food of uncontrolled quality, ingesting inadequate quantities for their needs. The aim of this work was to improve this picture. High pressure liquid chromatography (HPLC) was used to determine folic acid according to CATHARINO and GODOY (2000), in samples of sterilized milk produced in a pilot plant and also in samples purchased on the market, in powdered milk, ready to use milk chocolate beverage, margarine, flour and bread. Some modifications in the methodology wêre made, mainly in the extraction step, for the samples of flour and bread. These alterations were validated with respect to recover, and values of from 92 to 98% were obtained, repeatability was very well. The limits of detection and quantification being respectively 1,3ng/mL and 2,6ng/mL. Four sample were analysed in 3 different lots of fluid milk purchased on the market, and the values of folic acid were found to be below the values declared on the label of the products, in 50% of the samples. After 3 months, the loss of folic acid approached 20%. In all the 4 experiments with milk sterilization made in the pilot plant, the process of addition and homogenization resulted in a loss of folic acid of 54%, while the process of sterilization was responsible for only 10%. In a 90-day period the loss of folic acid was 16%. The same milk samples were submitted to a boiling process and the vitamin was show to be resistant, with a conservation of about 99%. As for powdered milk and ready to use milk chocolate beverage, 4 samples of each were also analysed, in 3 different lots, for each product purchased on the market. The content of folic acid found was practically equal that declared by.the manufacturers for the powdered milk. Nevertheless, after 1 year of storage, the loss of folic acid reached 60% ...Note: The complete abstract is available with the full electronic digital thesis or dissertations

ASSUNTO(S)

vitamina m estabilidade vitaminas - analise acido folinico cromatografia liquida de alta eficiencia

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