Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.

AUTOR(ES)
RESUMO

A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

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