Construction of a semisynthetic antibody library using trinucleotide oligos.
AUTOR(ES)
Braunagel, M
RESUMO
A semisynthetic antibody library composed of single chain Fv fragments (scFv) was constructed by replacing the heavy chain CDR3 region of a human scFv by a random sequence of eight amino acids using trinucleotide codons. After cloning into a phage display vector, an antibody library was generated with a complexity of 8 x 10(8) independent clones. The library was screened for binders to dinitrophenol, fluorescein isothiocyanate and 3-nitro-4-hydroxy-5-iodophenylacetic acid. scFv antibodies that specifically bound the antigen were obtained in each case.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=147064Documentos Relacionados
- A more efficient and specific strategy in the ablation of mRNA in Xenopus laevis using mixtures of antisense oligos.
- Construction of a deletion library using a mixture of 5'-truncated primers for inverse PCR (IPCR).
- Construction of a human chromosome 3 specific NotI linking library using a novel cloning procedure.
- cDNA library construction from small amounts of RNA using paramagnetic beads and PCR.
- Efficient construction of a large nonimmune phage antibody library: The production of high-affinity human single-chain antibodies to protein antigens
