Conserved Nodulation Genes in Rhizobium meliloti and Rhizobium trifolii
AUTOR(ES)
Fisher, Robert F.
RESUMO
Plasmids which contained wild-type or mutated Rhizobium meliloti nodulation (nod) genes were introduced into Nod−R. trifolii mutants ANU453 and ANU851 and tested for their ability to nodulate clover. Cloned wild-type and mutated R. meliloti nod gene segments restored ANU851 to Nod+, with the exception of nodD mutants. Similarly, wild-type and mutant R. meliloti nod genes complemented ANU453 to Nod+, except for nodCII mutants. Thus, ANU851 identifies the equivalent of the R. meliloti nodD genes, and ANU453 specifies the equivalent of the R. meliloti nodCII genes. In addition, cloned wild-type R. trifolii nod genes were introduced into seven R. meliloti Nod− mutants. All seven mutants were restored to Nod+ on alfalfa. Our results indicate that these genes represent common nodulation functions and argue for an allelic relationship between nod genes in R. meliloti and R. trifolii.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=241742Documentos Relacionados
- Interference between Rhizobium meliloti and Rhizobium trifolii nodulation genes: genetic basis of R. meliloti dominance.
- Nucleotide sequence of Rhizobium meliloti nodulation genes.
- Expression of Rhizobium trifolii early nodulation genes on maize and rice plants.
- Nodules are induced on alfalfa roots by Agrobacterium tumefaciens and Rhizobium trifolii containing small segments of the Rhizobium meliloti nodulation region.
- A Family of Activator Genes Regulates Expression of Rhizobium Meliloti Nodulation Genes