Consensus sequence for Escherichia coli heat shock gene promoters.

AUTOR(ES)

Cowing, D W

RESUMO

We have identified promoters for the Escherichia coli heat shock operons dnaK and groE and the gene encoding heat shock protein C62.5. Transcription from each promoter is heat-inducible in vivo, and each is recognized in vitro by RNA polymerase containing sigma 32, the sigma factor encoded by rpoH (htpR) but not by RNA polymerase containing sigma 70. We compared the sequences of the heat shock promoters and propose a consensus promoter sequence, having T-N-t-C-N-C-c-C-T-T-G-A-A in the -35 region and C-C-C-C-A-T-t-T-a in the -10 region. These sequences differ from the consensus sequence recognized by holoenzyme containing sigma 70, the major sigma in E. coli. We suggest that the accumulated consensus sequences of promoters recognized by alternate forms of holoenzyme are compatible with a model in which sigma recognizes only the -10 region of the promoter.

Documentos Relacionados

• A highly conserved sequence in yeast heat shock gene promoters.
• Analysis of consensus sequence patterns in Giardia cytoskeleton gene promoters.
• Plasmid vehicles for direct cloning of Escherichia coli promoters.
• Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX.
• The heat shock consensus sequence is not sufficient for hsp70 gene expression in Drosophila melanogaster.