Conjugational Genetic Exchange in the Hyperthermophilic Archaeon Sulfolobus acidocaldarius: Intragenic Recombination with Minimal Dependence on Marker Separation
AUTOR(ES)
Hansen, Josh E.
FONTE
American Society for Microbiology
RESUMO
In Sulfolobus acidocaldarius conjugation assays, recombinant frequency was relatively constant for marker separations from 1,154 bp down to about 50 bp and readily detectable at 10 bp. Three-factor crosses revealed little, if any, genetic linkage over distances of 500 to 600 bp, and large deletion mutants were good donors but poor recipients in matings. The results indicate that most intragenic recombination events occur at one of the mutations, not in the interval between them.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=543538Documentos Relacionados
- Characterization of Intragenic Recombination in a Hyperthermophilic Archaeon via Conjugational DNA Exchange
- A succinate dehydrogenase with novel structure and properties from the hyperthermophilic archaeon Sulfolobus acidocaldarius: genetic and biophysical characterization.
- Exchange of genetic markers at extremely high temperatures in the archaeon Sulfolobus acidocaldarius.
- Molecular Characteristics of Spontaneous Deletions in the Hyperthermophilic Archaeon Sulfolobus acidocaldarius
- Genetic responses of the thermophilic archaeon Sulfolobus acidocaldarius to short-wavelength UV light.
