Conformationally Correct Expression of Membrane-Anchored Toxoplasma gondii SAG1 in the Primitive Protozoan Giardia duodenalis

AUTOR(ES)

Marti, Matthias

FONTE

American Society for Microbiology

RESUMO

To explore the possibility of expressing membrane-anchored exodomains of heterologous surface antigens in Giardia, a chimeric construct containing the Toxoplasma gondii SAG1 gene was made. The Giardia system is shown here to provide a means of generating correctly folded chimeric surface proteins in a native and unmodified form.

Documentos Relacionados

• Conformationally appropriate expression of the Toxoplasma antigen SAG1 (p30) in CHO cells.
• Membrane-Anchored Peptide Inhibits Human Immunodeficiency Virus Entry
• Membrane-anchored Plakoglobins Have Multiple Mechanisms of Action in Wnt Signaling
• The Surface of Toxoplasma Tachyzoites Is Dominated by a Family of Glycosylphosphatidylinositol-Anchored Antigens Related to SAG1
• Tic40, a membrane-anchored co-chaperone homolog in the chloroplast protein translocon