Complex synthetic chemical libraries indexed with molecular tags.
AUTOR(ES)
Ohlmeyer, M H
RESUMO
Combinatorial methods of chemical synthesis allow the creation of molecular libraries having immense diversity. The utility of such libraries is dependent upon identifying the structures of the molecules so prepared. We describe the construction of a peptide combinatorial library, having 117,649 different members, synthesized on beads and indexed with inert chemical tags. These tags are used as a binary code to record the reaction history of each bead. The code can be read directly from a single bead by electron capture capillary gas chromatography. We demonstrate the correct selection of members of the library on the basis of binding to a monoclonal antibody.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=47893Documentos Relacionados
- Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags.
- A catalogue of genes in the cardiovascular system as identified by expressed sequence tags.
- "Libraries from libraries": chemical transformation of combinatorial libraries to extend the range and repertoire of chemical diversity.
- The identification of CD4+ T cell epitopes with dedicated synthetic peptide libraries
- Creating a false positive clone as an aid in screening libraries with synthetic oligonucleotides.