Complementation of Conjugation Functions of Streptomyces lividans Plasmid pIJ101 by the Related Streptomyces Plasmid pSB24.2
AUTOR(ES)
Pettis, Gregg S.
FONTE
American Society for Microbiology
RESUMO
A database search revealed extensive sequence similarity between Streptomyces lividans plasmid pIJ101 and Streptomyces plasmid pSB24.2, which is a deletion derivative of Streptomyces cyanogenus plasmid pSB24.1. The high degree of relatedness between the two plasmids allowed the construction of a genetic map of pSB24.2, consisting of putative transfer and replication loci. Two pSB24.2 loci, namely, the cis-acting locus for transfer (clt) and the transfer-associated korB gene, were shown to be capable of complementing the pIJ101 clt and korB functions, respectively, a result that is consistent with the notion that pIJ101 and the parental plasmid pSB24.1 encode highly similar, if not identical, conjugation systems.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=103605Documentos Relacionados
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- Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101 and correlation of the sequence with genetic properties.
- Mutational Analysis of the tra Locus of the Broad-Host-Range Streptomyces Plasmid pIJ101
- Plasmid transfer in Streptomyces lividans: identification of a kil-kor system associated with the transfer region of pIJ101.
- Expression Characteristics of the Transfer-Related kilB Gene Product of Streptomyces Plasmid pIJ101: Implications for the Plasmid Spread Function
