Competitive Inhibition of Ferrous Iron Oxidation by Thiobacillus ferrooxidans by Increasing Concentrations of Cells

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The oxidation of ferrous iron (Fe2+) to ferric iron (Fe3+) with dioxygen (O2) by various strains of Thiobacillus ferrooxidans was studied by measuring the rate of O2 consumption at various Fe2+ concentrations and cell concentrations. The apparent Km values for Fe2+ remained constant at different cell concentrations of laboratory strains ATCC 13661 and ATCC 19859 but increased with increasing cell concentrations of mine isolates SM-4 and SM-5. The latter results are explained by the competitive inhibition of the Fe2+-binding site of a cell by other cells in the reaction mixture. Possible mechanisms involving cell surface properties are discussed.

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