Competence-Inducing Factor of Bacillus stearothermophilus1

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RESUMO

An incompetent mutant (4S Com−) does not release competence-inducing factor (CF) into the culture medium and is not infected with TP-1C phage deoxyribonucleic acid (DNA) unless CF is added to the transfection assay. A TP-1C phage-resistant mutant (4S Ton-r) releases relatively large amounts of CF into the culture medium but is not infected with TP-1C phage DNA, even in the presence of CF. The production of CF by the wild type or Ton-r mutant and the ability of the wild type or Com− mutant to react with CF does not occur after these cultures have grown at 67 C for 1 hr or longer. A preliminary characterization of the CF is described. The autolytic enzyme or the temperate phage of the wild type and the Ton-r and Com− mutants do not have competence-inducing activity.

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