Compartmentation in Vicia faba Leaves: I. Kinetics of 14C in the Tissues following Pulse Labeling 12
AUTOR(ES)
Outlaw, William H.
RESUMO
Leaflets of Vicia faba were pulse-labeled with 14CO2 to follow the subsequent movement of photosynthate between leaf tissues. Samples were taken during a 12CO2 chase, quick frozen, freeze-substituted, and embedded in methacrylate. Paradermal sections provided tissue samples consisting only of upper epidermis, palisade parenchyma, spongy parenchyma and veins, spongy parenchyma, or lower epidermis. Most CO2 fixation occurred in the palisade parenchyma, but its 14C content declined rapidly after labeling. Concomitant with the decline of activity in the palisade parenchyma, there was an increase in activity in the spongy parenchyma and upper epidermis and a slow increase in the lower epidermis. Activity in the palisade parenchyma and spongy parenchyma eventually reached similar levels and remained constant. Tissue samples containing veins were consistently the most radioactive, and activity in those samples showed a decline. Very little change occurred in the insoluble fraction from any tissue. The results support previous assumptions regarding the pathway of assimilate transport to the veins, and demonstrate the rapidity of such transport. Sucrose is apparently the principal mobile compound.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541689Documentos Relacionados
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