Comparison of three reagents for detecting indole production by anaerobic bacteria in microtest systems.
AUTOR(ES)
Lombard, G L
RESUMO
Three reagents for detecting indole, Kovac, Ehrlich, and p-dimethylaminocinnamaldehyde (DMCA), were evaluated with commercial microtest systems for characterizing and identifying anaerobic bacteria. The DMCA reagent, the most sensitive of the three reagents, gave a positive reaction with 445 of 449 strains of various indole-producing anaerobic bacteria. There was 99.6% agreement between the results obtained with the DMCA in the microtest systems and results using the conventional tube test to detect indole by using xylene extraction and Ehrlich reagent. Ehrlich reagent detected indole in 163 of 176 (92.6%) indole-positive strains when the inoculum was overlaid with mineral oil before incubation. Kovac reagent was the least sensitive of the reagents tested. When the inoculum was overlaid with mineral oil, Kovac reagent detected only 80 of 108 (74.0%) of indole-positive strains. In addition to being the most sensitive reagent for detection indole, DMCA also allowed detection of indole derivatives (skatole, 3-indolepropionic acid, and 3-indolebutyric acid) produced by some clostridia.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=270862Documentos Relacionados
- Recovery of anaerobic, facultative, and aerobic bacteria from clinical specimens in three anaerobic transport systems.
- Survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems.
- Gelatin agar medium for detecting gelatinase production by anaerobic bacteria.
- Comparison of three procedures for biochemical testing of anaerobic bacteria.
- Methods for Detecting Indole Production by Gram-Negative Nonsporeforming Anaerobes
