Comparison of the polypeptide composition of Escherichia coli outer membranes prepared by two methods.
AUTOR(ES)
Chopra, I
RESUMO
Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The patterns obtained were not identical, and Sarkosyl removed several minor proteins from the outer membrane.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=294674Documentos Relacionados
- Comparison of two biological aerosol sampling methods.
- Calculating expected deaths: a comparison of two methods.
- Comparison of the Pasteurella haemolytica A1 envelope proteins obtained by two cell disruption methods.
- Effect of growth medium on the relative polypeptide composition of cellular outer membrane and released outer membrane material in Escherichia coli.
- A comparison of serum folate estimations using two different methods.
