Comparison of TestPack Strep A test kit with culture technique for detection of group A streptococci.
AUTOR(ES)
Schwabe, L D
RESUMO
Results obtained with Abbott Laboratories TestPack Strep A (TPSA), a 7-min enzyme immunoassay method, were compared with culture results to measure the ability of this assay to detect group A streptococci directly from 365 throat swabs. Our study demonstrated a sensitivity of 90.0% and a specificity of 97.4% for TPSA compared with cultures incubated for 48 h. The positive and negative predictive values of this assay versus the culture method were 92.8 and 96.3%, respectively. If specimens that provided fewer than 10 colonies per plate of group A streptococci are eliminated from the data, the sensitivity is increased to 95.6%. Additionally, 10 group A and 40 non-group A streptococcal isolates were tested directly with TPSA for the ability to distinguish group A from non-group A streptococci. All 50 isolates were correctly identified (100% accuracy). TPSA is a rapid, accurate, and easy-to-interpret method for detection and confirmation of group A streptococcal antigen directly from throat swabs and pure culture isolates.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265889Documentos Relacionados
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