Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay.
AUTOR(ES)
Bromberg, K
RESUMO
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection in children. Rapid identification of RSV infections would allow for specific chemotherapy. We evaluated a means of rapid diagnosis, the Abbott enzyme immunoassay (EIA), by using 314 stored nasopharyngeal aspirates. RSV antigens were identified in 62 of 66 RSV culture-positive specimens. An additional 37 specimens from which RSV was not isolated were positive in the EIA. Of these, 29 were confirmed as truly positive by a blocking assay, for a total of 95 (66 + 29) positive specimens. The sensitivity of the EIA for total positive samples was 96% (91/95) versus 69% (66/95) for cell culture. The specificity of the EIA was 96% (211/219). In these stored specimens, Abbott EIA was superior to cell culture for the detection of RSV.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265918Documentos Relacionados
- Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture.
- Detection of respiratory syncytial virus antigen in nasopharyngeal secretions by Abbott Diagnostics enzyme immunoassay.
- Detection of respiratory syncytial virus antigen in nasal washings by Abbott TestPack enzyme immunoassay.
- Comparison of immediate and delayed inoculation of HEp-2 cells for isolation of respiratory syncytial virus.
- Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay.
