Comparative susceptibilities of strain MRC-5 human embryonic lung fibroblast cells and the Cooney strain of human fetal tonsil cells for isolation of rhinoviruses from clinical specimens.
AUTOR(ES)
Geist, F C
RESUMO
The comparative susceptibilities for rhinovirus isolation were determined for strain MRC-5 human embryonic lung fibroblast and human fetal tonsil cells. Samples from nasopharyngeal and throat swab clinical specimens were inoculated onto duplicate monolayers of each cell type. Of 105 rhinovirus-positive specimens, 78 (74%) were positive in MRC-5 cells, and 102 (97%) were positive in fetal tonsil cells (P less than 0.001). For 75 specimens positive in both, the mean time to cytopathic effect development with standard deviation was 1 +/- 2 days shorter in fetal tonsil cells (P less than 0.01). The use of a second virus culture increased rhinovirus isolations by 10% compared with a single culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=268434Documentos Relacionados
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