Comparative study on the mechanisms of rotavirus inactivation by sodium dodecyl sulfate and ethylenediaminetetraacetate.
AUTOR(ES)
Ward, R L
RESUMO
This report describes a comparative study on the effects of the anionic detergent sodium dodecyl sulfate and the chelating agent ethylenediaminetetraacetate on purified rotavirus SA-11 particles. Both chemicals readily inactivated rotavirus at quite low concentrations and under very mild conditions. In addition, both agents modified the viral capsid and prevented the adsorption of inactivated virions to cells. Capsid damage by ethylenediaminetetraacetate caused a shift in the densities of rotavirions from about 1.35 to about 1.37 g/ml and a reduction in their sedimentation coefficients. Sodium dodecyl sulfate, on the other hand, did not detectably alter either of these physical properties of rotavirions. Both agents caused some alteration of the isoelectric points of the virions. Finally, analysis of rotavirus proteins showed that ethylenediaminetetraacetate caused the loss of two protein peaks from the electrophoretic pattern of virions but sodium dodecyl sulfate caused the loss of only one of these same protein peaks.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=291498Documentos Relacionados
- Comparative Study of Mechanisms of Herpes Simplex Virus Inactivation by Sodium Lauryl Sulfate and n-Lauroylsarcosine
- Enzymes Induced in a Bacterium by Growth on Sodium Dodecyl Sulfate
- Effects of Treatment with Sodium Dodecyl Sulfate on the Ultrastructure of Escherichia coli
- Extraction of rotavirus from human feces by treatment with lithium dodecyl sulfate.
- Curing Action of Sodium Dodecyl Sulfate on a Proteus mirabilis R+ Strain