Comparative study of ribosomal genes activity in leukocytes and gingival tissues from Down syndrome individuals, in relation to age. / Estudo comparativo da atividade dos genes ribossômicos em mucosa bucal (gengiva) e leucócitos de indivíduos com Síndrome de Down em relação à idade.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Cytogenetic and molecular studies have shown significant reduction in the ribosomal genes activity in lymphocyte and fibroblasts cells from older Trisomy 21 or Down syndrome (DS) individuals as well as in lymphocyte and neural cortical cells from Alzheimers disease patients. We have proposed the study of rRNA levels in older and younger DS subjects in two different cell tissues, blood and gingival cells. Gingival cells share the same neuroectodermal origin as neural cells and should indicate common properties and/or functions. The aim of the study was to analyze rDNA activity through rRNA 28S/18S ratio levels in blood and gingival cells from younger and older DS subjects. rRNA 28S/18S ratio levels were investigated in both tissues from ten younger (6-21 years old) and ten older DS subjects (35-54 years old) and in the same age and sex controls through the Northern Blotting method. Blood cells rRNA levels were also compared between 42 DS subjects and 42 controls. The results showed no significant difference in rRNA 28S/18S ratios between gingival and blood tissues from younger and older DS as well as from younger and elderly controls. Therefore the transcriptional regulation of rDNA through Northern blotting technique in blood and gingival cells from younger and older DS cells and from healthy controls has not been considered a marker for a pre clinical status for Alzheimers disease in DS subjects. Furthermore, the common embryonic origin between neural and gingival cells neither influenced ribosomal genes activity considering their different proliferation rates nor the potential presence of some stem cells in gingiva.

ASSUNTO(S)

1. síndrome de down, 2. rna ribossômico, 3. genes ribossômicos, 4. envelhecimento morfologia

ACESSO AO ARTIGO

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