Comparative studies on the effect of growth conditions on adhesion, hydrophobicity, and extracellular protein profile of Streptococcus sanguis G9B.

AUTOR(ES)

Knox, K W

RESUMO

Streptococcus sanguis G9B was grown in continuous culture at different generation times and pH values in media containing either glucose or fructose and differing in the concentrations of Na+ and K+. The growth pH, carbohydrate, and cation concentration each affected the yield of organisms, their ability to adhere to saliva-coated hydroxyapatite beads, and their hydrophobicity, as measured by adhesion to hexadecane. There was no correlation between adhesion to saliva-coated hydroxyapatite beads and hydrophobicity, the values for hydrophobicity varying between 44 and 83% for organisms that adhered poorly and between 24 and 75% for those that adhered effectively. For organisms grown in batch culture at pH 6.0 or 7.0 there was similarly no correlation between adhesion and hydrophobicity. The growth conditions also had a considerable influence on the production of extracellular protein. The total amount was greater at pH 7.5 than at other pH values, and there were also differences in the individual components in response to changes in generation time, pH, carbohydrate source, and cation concentration. Two protein bands were identified, namely, glucosyltransferase and protein P1 (also called antigen B or I/II). However, there was no correlation between a particular protein component and adhesion.

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