Comparative Studies on Microbial and Chemical Modifications of Trichothecene Mycotoxins
AUTOR(ES)
Yoshizawa, Takumi
RESUMO
The microbial modification of several trichothecene mycotoxins by trichothecene-producing strains of Fusarium nivale and F. solani was studied. These results were compared with the corresponding chemical modifications. The growing mycelia of Fusarium spp. did not convert 4β-acetoxy-3α,7α,15-trihydroxy-12,13-epoxytrichothec-9-en-8-one (fusarenon) into 3α,4β,7α,15-tetrahydroxy-12,13-epoxy-trichothec-9-en-8-one (nivalenol), whereas 3α,4β,7α,15-tetraacetoxy-12,13-epoxytrichothec-9-en-8-one (tetraacetylnivalenol) was deacetylated to yield 3α-hydroxy-4β,7α,15-triacetoxy-12,13-epoxytrichothec-9-en-8-one (4,7,15-triacetylnivalenol), which was resistant to further deacetylation. T-2 toxin was transformed into HT-2 toxin, and 8α-(3-methylbutyryloxy)-3α,4β,-15-triacetoxy-12,13-epoxytrichothec-9-en-8-one (T-2 acetate) was transformed into HT-2 toxin via T-2 toxin. Chemical modification with ammonium hydroxide converted tetraacetylnivalenol into fusarenon via 4,7,15-triacetylnivalenol. 3α,-7α,15-Triacetoxy-12, 13-epoxytrichothec-9-en-8-one (triacetyldeoxynivalenol) gave deacetylation products lacking the C-7 or C-15 acetyl group in addition to 7α,15 - diacetoxy - 3α - hydroxy - 12,13 - epoxytrichothec - 9 - en - 8 - one (7,15 - diacetyldeoxynivalenol). These results demonstrate the regio-selectivity in microbial modification of trichothecenes. Based on the results and available knowledge concerning the transformation of trichothecenes, mechanisms for biological modifications of these mycotoxins are postulated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=187110Documentos Relacionados
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