Comparative methods for detection of thymidine kinase-deficient herpes simplex virus type 1 strains.
AUTOR(ES)
Harmenberg, J
RESUMO
Four methods for analyzing viral susceptibility to antiviral substances were compared. In two methods viral products were measured: late viral proteins were measured by an enzyme-linked immunosorbent assay and viral DNA was measured by DNA hybridization. Infectious virus was quantified in the other two assays as the number of plaques and the yield of virus. The enzyme-linked immunosorbent assay procedure in our hands detected the smallest amounts (lowest proportions) of thymidine kinase-deficient herpes simplex virus type 1 mixed with wild-type virus. The thymidine kinase-deficient proportion of the herpes simplex virus type 1 isolate increased rapidly in the presence of acyclovir in cell culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=176482Documentos Relacionados
- Thymidine kinase-deficient herpes simplex virus type 2 genital infection in guinea pigs.
- Latent infections in spinal ganglia with thymidine kinase-deficient herpes simplex virus.
- Plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates.
- Herpes simplex virus thymidine kinase activity of thymidine kinase-deficient Escherichia coli K-12 mutant transformed by hybrid plasmids.
- Herpes Simplex Virus as a Source of Thymidine Kinase for Thymidine Kinase-Deficient Mouse Cells: Suppression and Reactivation of the Viral Enzyme