Comparative Evaluation of First- and Second-Generation Digene Hybrid Capture Assays for Detection of Human Papillomaviruses Associated with High or Intermediate Risk for Cervical Cancer

AUTOR(ES)

Poljak, Mario

FONTE

American Society for Microbiology

RESUMO

In the present study we comparatively evaluated the first- and second-generation Digene Hybrid Capture assays for detection of human papillomaviruses (HPV) associated with high or intermediate risk for cervical cancer in cervical specimens. Concordant results were obtained with 468 of 483 (96.8%) specimens. All 15 specimens which gave repeatedly discordant results were positive by the second-generation test only, and 14 of them tested PCR positive. The enhanced sensitivity of the second-generation assay is mainly a result of the reformulation of hybridization reagents and, to a lesser extent, a result of the addition of new HPV probes.

Documentos Relacionados

• Evaluation of first- and second-generation RIBA kits for detection of antibody to hepatitis C virus.
• First- and second-generation total synthesis of ciguatoxin CTX3C
• Comparison of the Second-Generation Digene Hybrid Capture Assay with the Branched-DNA Assay for Measurement of Hepatitis B Virus DNA in Serum
• Health and literacy in first- and second-generation Moroccan Berber women in the Netherlands: Ill literacy?
• Elevated mutation rates in the germ line of first- and second-generation offspring of irradiated male mice