Comparative cytogenetics of three species of Dichotomius (Coleoptera, Scarabaeidae)

AUTOR(ES)

Silva, Guilherme Messias da, Bione, Edgar Guimarães, Cabral-de-Mello, Diogo Cavalcanti, Moura, Rita de Cássia de, Simões, Zilá Luz Paulino, Souza, Maria José de

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

01/05/2009

RESUMO

Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in autosomes of D. semisquamosus and D. sericeus and in the sexual bivalent of D. nisus. FISH with an rDNA probe confirmed NORs location in D. semisquamosus and in D. nisus. Our results suggest that chromosome inversions and fusions occurred during the evolution of the group.

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