Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
AUTOR(ES)
Leal, C.A.G., Carvalho-Castro, G.A., Cottorello, A.C., Leite, R.C., Figueiredo, H.C.P.
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
15/11/2013
RESUMO
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
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