Combined single-molecule force and fluorescence measurements for biology

AUTOR(ES)

Wallace, Mark I

FONTE

BioMed Central

RESUMO

Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution.

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