Coding capacity of complementary DNA strands.
AUTOR(ES)
Casino, A
RESUMO
A Fortran computer algorithm has been used to analyze the nucleotide sequence of several structural genes. The analysis performed on both coding and complementary DNA strands shows that whereas open reading frames shorter than 100 codons are randomly distributed on both DNA strands, open reading frames longer than 100 codons ("virtual genes") are significantly more frequent on the complementary DNA strand than on the coding one. These "virtual genes" were further investigated by looking at intron sequences, splicing points, signal sequences and by analyzing gene mutations. On the basis of this analysis coding and complementary DNA strands of several eukaryotic structural genes cannot be distinguished. In particular we suggest that the complementary DNA strand of the human epsilon-globin gene might indeed code for a protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=326772Documentos Relacionados
- Statistical evaluation of the coding capacity of complementary DNA strands.
- Separation of B. subtilis DNA into complementary strands. 3. Direct analysis.
- Nucleotide sequences of the separate origins of synthesis of bacteriophage G4 viral and complementary DNA strands.
- Seapration of B. subtilis DNA into complementary strands. II. Template functions and composition as determined by transcription with RNA polymerase.
- Vertebrate histone gene transcription occurs from both DNA strands.
