Cocultivation of the amoeba Naegleria fowleri and the amoebicin- producing strain Bacillus licheniformis M-4.
AUTOR(ES)
Lebbadi, M
RESUMO
Antagonism between Bacillus licheniformis M-4 and the pathogenic amoeba Naegleria fowleri HB-1 during cocultivation was influenced by the composition of the medium and the initial amoeba/bacterium ratio. While a ratio of 50 caused complete lysis of amoebae in soil extract with 0.3% glucose (SEG) before 72 h, this ratio had to be at least 12-fold lower in order to obtain similar results in Cline medium. Sporulation of B. licheniformis M-4 took place much earlier in SEG. Amoebicin production was stimulated by the presence of amoebae by either shortening the time of production (as in SEG) or increasing the amount of amoebicins released (as in Cline medium). Electron microscopy showed that amoebae cocultivated in the Cline medium contained bacteria enclosed in digestive vacuoles, while amoebae from SEG cocultures did not.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=167426Documentos Relacionados
- Biological activity of amoebicin m4-A from Bacillus licheniformis M-4.
- Sucker-like structures on the pathogenic amoeba Naegleria fowleri.
- Genetic Variation in the Free-Living Amoeba Naegleria fowleri
- A multicomponent hemolytic system in the pathogenic amoeba Naegleria fowleri.
- Characterization and biological activity against Naegleria fowleri of amoebicins produced by Bacillus licheniformis D-13.
